Anti-Phospho-PKD2 (Ser876) PRKD2 Antibody

Protein Kinase D2 antibody

Boster Bio Anti-Phospho-PKD2 (Ser876) PRKD2 Antibody (Catalog # P04056). Tested in WB, IHC, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: P04056
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-Phospho-PKD2 (Ser876) PRKD2 Antibody

View all Protein Kinase D2 Antibodies

SKU/Catalog Number

P04056

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-PKD2 (Ser876) PRKD2 Antibody (Catalog # P04056). Tested in WB, IHC, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-PKD2 (Ser876) PRKD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P04056)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Immunogen

Synthesized peptide derived from human PKD2 around the phosphorylation site of S876.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P04056 is reactive to PRKD2 in Human, Mouse, Rat

Applications

P04056 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

96.722kDa

Background of Protein Kinase D2

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.

Ferrer, et al. (2003) Neuropathology & Applied Neurobiology 29: 23
Zhonghong Guan, et al. (1999) J Biol Chem, Vol. 274: 36200-36206
D.Margriet Ouwens1, et al. (2002)The EMBO Journal 21: 3782

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1/500 - 1/2000
Immunohistochemistry: 1/100 - 1/300
ELISA: 1/40000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For PRKD2 (Source: Uniprot.org, NCBI)

Gene Name

PRKD2

Full Name

Serine/threonine-protein kinase D2

Weight

96.722kDa

Superfamily

protein kinase superfamily

Alternative Names

DKFZP586E0820; EC 2.7.11; EC 2.7.11.13; nPKC-D2; PKD2; PKD2HSPC187; PRKD2; protein kinase D2; serine/threonine-protein kinase D2 PRKD2 HSPC187, PKD2, nPKC-D2 protein kinase D2 serine/threonine-protein kinase D2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PRKD2, check out the PRKD2 Infographic

PRKD2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRKD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Phospho-PKD2 (Ser876) PRKD2 Antibody

Question

Can you help my question with product P04056, anti-Phospho-PKD2 (Ser876) Polyclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-11-11

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P04056 anti-Phospho-PKD2 (Ser876) Polyclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-11

Question

Is this P04056 anti-Phospho-PKD2 (Ser876) Polyclonal antibody reactive to the isotypes of PRKD2?

Verified Customer

Verified customer

Asked: 2018-06-27

Answer

The immunogen of P04056 anti-Phospho-PKD2 (Ser876) Polyclonal antibody is Synthesized peptide derived from human PKD2 around the phosphorylation site of S876. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-06-27

Question

We are currently using anti-Phospho-PKD2 (Ser876) Polyclonal antibody P04056 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?

C. Brown

Verified customer

Asked: 2014-02-14

Answer

The anti-Phospho-PKD2 (Ser876) Polyclonal antibody (P04056) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-02-14

Question

Will anti-Phospho-PKD2 (Ser876) Polyclonal antibody P04056 work on pig WB with umbilical cord blood?

E. Walker

Verified customer

Asked: 2013-04-11

Answer

Our lab technicians have not tested anti-Phospho-PKD2 (Ser876) Polyclonal antibody P04056 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Phospho-PKD2 (Ser876) Polyclonal antibody P04056 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig umbilical cord blood in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-04-11

Order DetailsPrice
P04056-100uL

100uL

$360

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P04056
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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